Fibroblasts/Epithelial cells Antibody (D7-FIB) [Alexa Fluor™ Plus 647]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-777AFP647
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation, Immunohistochemistry-Paraffin (Negative)
Label
Alexa Fluor Plus 647
Antibody Source
Monoclonal Mouse IgG2A Clone # D7-FIB
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human foreskin fibroblasts
Localization
Cell Membrane
Specificity
NB600-777 recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (3). This product is routinely tested in flow cytometry on the KG1 cell line.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin (Negative)
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Fibroblasts/Epithelial cells
Alternate Names
fibro-blasts
Additional Fibroblasts/Epithelial cells Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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