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FGFR4 Antibody (240929) [Allophycocyanin]

Catalog # FAB6852A | Novus Biologicals a Bio-Techne Brand
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FAB6852A-0.1ml

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 240929

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for FGFR4 Antibody (240929) [Allophycocyanin]

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FGF R4
Leu22-Asp369 (predicted)
Accession # P22455

Specificity

Detects human FGF R4 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse FGF R4 and recombinant human FGF R5 is observed and no cross-reactivity with any isoform of rhFGF R1, rhFGF R2, or rhFGF R3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for FGFR4 Antibody (240929) [Allophycocyanin]

Detection of FGF R4 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of FGF R4 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Rat Anti-Human FGF R4 APC-conjugated Monoclonal Antibody (Catalog # FAB6852A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for FGFR4 Antibody (240929) [Allophycocyanin]

Application
Recommended Usage

Flow Cytometry

10 uL/10^6 cells
Sample: See below
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using FAB6852A in the following applications:

Published Applications

Read 1 publication using FAB6852A in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Preservative

Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FGFR4

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1‑4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1‑3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. Only the alpha isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1‑3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1‑3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.

References

  1. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
  2. Burke, D. et al. (1998) Trends Biochem. Sci. 23:59.

Long Name

Fibroblast Growth Factor Receptor 4

Alternate Names

CD334, FGF R4

Gene Symbol

FGFR4

UniProt

Product Documents for FGFR4 Antibody (240929) [Allophycocyanin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FGFR4 Antibody (240929) [Allophycocyanin]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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