FAK [p Tyr407] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13085
![Western Blot: FAK [p Tyr407] Antibody [NBP3-13085]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr407]-Antibody-Western-Blot-NBP3-13085-img0003.jpg "Western Blot: FAK [p Tyr407] Antibody [NBP3-13085]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr407 of human FAK. The exact sequence is proprietary.
Modification
p Tyr 407
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for FAK [p Tyr407] Antibody
![Western Blot: FAK [p Tyr407] Antibody [NBP3-13085]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr407]-Antibody-Western-Blot-NBP3-13085-img0005.jpg "Western Blot: FAK [p Tyr407] Antibody [NBP3-13085]")
Western Blot: FAK [p Tyr407] Antibody [NBP3-13085]
Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:500.![Immunocytochemistry/ Immunofluorescence: FAK [p Tyr407] Antibody [NBP3-13085]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr407]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13085-img0004.jpg "Immunocytochemistry/ Immunofluorescence: FAK [p Tyr407] Antibody [NBP3-13085]")
Immunocytochemistry/ Immunofluorescence: FAK [p Tyr407] Antibody [NBP3-13085]
Immunocytochemistry/Immunofluorescence: FAK [p Tyr407] Antibody [NBP3-13085] - FAK (phospho Tyr407) antibody detects FAK (phospho Tyr407) protein at focal adhesion and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FAK (phospho Tyr407) stained by FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.![Western Blot: FAK [p Tyr407] Antibody [NBP3-13085]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr407]-Antibody-Western-Blot-NBP3-13085-img0002.jpg "Western Blot: FAK [p Tyr407] Antibody [NBP3-13085]")
Western Blot: FAK [p Tyr407] Antibody [NBP3-13085]
Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![FAK [p Tyr407] Antibody](https://resources.bio-techne.com/images/products/nbp3-13085_rabbit-fak-p-tyr-407-pab-western-blot-261120251829012.jpg "Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] -")
![FAK [p Tyr407] Antibody](https://resources.bio-techne.com/images/products/nbp3-13085_rabbit-fak-p-tyr-407-pab-western-blot-2611202519483.jpg "Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] -")
![FAK [p Tyr407] Antibody](https://resources.bio-techne.com/images/products/nbp3-13085_rabbit-fak-p-tyr-407-pab-western-blot-261120251971814.jpg "Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] -")
Applications for FAK [p Tyr407] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FAK
FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness.
Long Name
Focal adhesion kinase 1
Alternate Names
FADK1, PTK2
Gene Symbol
PTK2
Additional FAK Products
Product Specific Notices for FAK [p Tyr407] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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