FAK [p Tyr397] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12897
![FAK [p Tyr397] Antibody](https://resources.bio-techne.com/images/products/nbp3-12897_rabbit-fak-p-tyr-397-pab-910202419593568.jpg "Immunocytochemistry/ Immunofluorescence: FAK [p Tyr397] Antibody [NBP3-12897] -")
![Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0001.jpg "Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]")
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr397 of human FAK. The exact sequence is proprietary.
Modification
p Tyr397
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
119 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for FAK [p Tyr397] Antibody
![Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0002.jpg "Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]")
Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]
Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0006.jpg "Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]")
Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]
Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HUVEC whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![FAK [p Tyr397] Antibody](https://resources.bio-techne.com/images/products/nbp3-12897_rabbit-fak-p-tyr-397-pab-western-blot-261120251843458.jpg "Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] -")
![FAK [p Tyr397] Antibody](https://resources.bio-techne.com/images/products/nbp3-12897_rabbit-fak-p-tyr-397-pab-western-blot-261120251850307.jpg "Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] -")
![FAK [p Tyr397] Antibody](https://resources.bio-techne.com/images/products/nbp3-12897_rabbit-fak-p-tyr-397-pab-western-blot-2611202518454017.jpg "Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] -")
![FAK [p Tyr397] Antibody](https://resources.bio-techne.com/images/products/nbp3-12897_rabbit-fak-p-tyr-397-pab-western-blot-2611202518381522.jpg "Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] -")
![FAK [p Tyr397] Antibody](https://resources.bio-techne.com/images/products/nbp3-12897_rabbit-fak-p-tyr-397-pab-western-blot-261120251823537.jpg "Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] -")
Applications for FAK [p Tyr397] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FAK
FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness.
Long Name
Focal adhesion kinase 1
Alternate Names
FADK1, PTK2
Gene Symbol
PTK2
Additional FAK Products
Product Specific Notices for FAK [p Tyr397] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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