FAK Antibody (OTI4D11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45923
![Immunocytochemistry/ Immunofluorescence: FAK Antibody (OTI4D11) [NBP2-45923]](https://resources.bio-techne.com/images/products/FAK-Antibody-OTI4D11-Immunocytochemistry-Immunofluorescence-NBP2-45923-img0001.jpg "Immunocytochemistry/ Immunofluorescence: FAK Antibody (OTI4D11) [NBP2-45923]")
![Western Blot: FAK Antibody (OTI4D11) [NBP2-45923]](https://resources.bio-techne.com/images/products/FAK-Antibody-OTI4D11-Western-Blot-NBP2-45923-img0008.jpg "Western Blot: FAK Antibody (OTI4D11) [NBP2-45923]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4D11
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PTK2(NP_722560) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
119.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FAK Antibody (OTI4D11)
Western Blot: FAK Antibody (OTI4D11) [NBP2-45923]
Western Blot: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAK .![Immunocytochemistry/ Immunofluorescence: FAK Antibody (OTI4D11) [NBP2-45923]](https://resources.bio-techne.com/images/products/FAK-Antibody-OTI4D11-Immunocytochemistry-Immunofluorescence-NBP2-45923-img0002.jpg "Immunocytochemistry/ Immunofluorescence: FAK Antibody (OTI4D11) [NBP2-45923]")
Immunocytochemistry/ Immunofluorescence: FAK Antibody (OTI4D11) [NBP2-45923]
Immunocytochemistry/Immunofluorescence: FAK Antibody (OTI4D11) [NBP2-45923] - FAK Antibody (4D11) [NBP2-45923] - Analysis of HeLa cells.![Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923]](https://resources.bio-techne.com/images/products/FAK-Antibody-OTI4D11-Immunohistochemistry-NBP2-45923-img0007.jpg "Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923]")
Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923]
Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923]](https://resources.bio-techne.com/images/products/FAK-Antibody-OTI4D11-Immunohistochemistry-NBP2-45923-img0003.jpg "Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923]")
![Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923]](https://resources.bio-techne.com/images/products/FAK-Antibody-OTI4D11-Immunohistochemistry-NBP2-45923-img0004.jpg "Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923]")
![Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923]](https://resources.bio-techne.com/images/products/FAK-Antibody-OTI4D11-Immunohistochemistry-NBP2-45923-img0005.jpg "Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923]")
![Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923]](https://resources.bio-techne.com/images/products/FAK-Antibody-OTI4D11-Immunohistochemistry-NBP2-45923-img0006.jpg "Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923]")
Applications for FAK Antibody (OTI4D11)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:4000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FAK
FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness.
Long Name
Focal adhesion kinase 1
Alternate Names
FADK1, PTK2
Gene Symbol
PTK2
Additional FAK Products
Product Documents for FAK Antibody (OTI4D11)
Product Specific Notices for FAK Antibody (OTI4D11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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