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Exonuclease 1 Antibody (HL3547)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48825

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-48825

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3547

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragment of human Exonuclease 1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Exonuclease 1 Antibody (HL3547) (NBP3-48825) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Exonuclease 1 Antibody (HL3547)

Exonuclease 1 Antibody (HL3547)

Immunocytochemistry/ Immunofluorescence: Exonuclease 1 Antibody (HL3547) [NBP3-48825] -

Exonuclease 1 antibody [HL3547] detects Exonuclease 1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Exonuclease 1 stained by Exonuclease 1 antibody [HL3547] (NBP3-48825) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Exonuclease 1 Antibody (HL3547)

Western Blot: Exonuclease 1 Antibody (HL3547) [NBP3-48825] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Exonuclease 1 antibody [HL3547] (NBP3-48825) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Exonuclease 1 Antibody (HL3547)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Exonuclease 1

Exonuclease 1 encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.

Alternate Names

Exo1, EXOI, Exonuclease I

Gene Symbol

EXO1

Additional Exonuclease 1 Products

Product Documents for Exonuclease 1 Antibody (HL3547)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Exonuclease 1 Antibody (HL3547)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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