EphA3 Antibody (IIIA4) [Janelia Fluor® 669] - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48887JF669

Recombinant Monoclonal Antibody

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vivo assay, Surface Plasmon Resonance

Label

Janelia Fluor 669

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # IIIA4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EphA3 Antibodies »

Product Specifications

Immunogen

The original antibody was prepared by intraperitoneal immunization of a BALB/c mouse with LK63 cells (Uniprot#: P29320)

Specificity

The antibody binds to the native EphA3 globular ephrin-binding domain. The antibody targets a site closely adjacent to the heterotetramerization site on the N-terminal of EPHA3's extracellular domain adjacent to the ligand-binding site and has a high affinity for EPHA3.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for EphA3 Antibody (IIIA4) [Janelia Fluor® 669] - Chimeric

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

In vivo assay

Optimal dilutions of this antibody should be experimentally determined.

Surface Plasmon Resonance

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP3-48887. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

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Plan Your Experiments

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: EphA3

Eph receptor A3 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.

Long Name

Eph Receptor A3

Alternate Names

Cek4, Hek4, Tyro4

Additional EphA3 Products

Product Documents for EphA3 Antibody (IIIA4) [Janelia Fluor® 669] - Chimeric

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EphA3 Antibody (IIIA4) [Janelia Fluor® 669] - Chimeric



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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