Key Product Details

Species Reactivity

Human

Applications

Western Blot, Adhesion Blockade

Label

HRP

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all E-Selectin/CD62E Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human E-Selectin

Specificity

Selected for its ability to inhibit the block U937 cell adhesion to immobilized rhE-Selectin. In direct ELISAs, less than 5% cross-reactivity with rmE-Selectin and rrE-Selectin is observed and less than 1% cross-reactivity with rhP-Selectin and rhL-Selectin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for E-Selectin/CD62E Antibody [HRP]

Application
Recommended Usage

Adhesion Blockade

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product AF724. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: E-Selectin/CD62E

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and to be useful for flow cytometry.

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Gene Symbol

SELE

Additional E-Selectin/CD62E Products

Product Documents for E-Selectin/CD62E Antibody [HRP]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for E-Selectin/CD62E Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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