E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07903

Recombinant Rabbit Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # CDH1/2208R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all E-Cadherin Antibodies »

Product Specifications

Immunogen

Recombinant full-length human E-Cadherin protein (Uniprot: P12830)

Localization

Cell Surface

Marker

Intercellular Junction Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07902).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free

Western Blot: E-Cadherin Antibody (CDH1/2208R)Azide and BSA Free [NBP3-07903]

Western Blot: E-Cadherin Antibody (CDH1/2208R)Azide and BSA Free [NBP3-07903]

Western Blot: E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free [NBP3-07903] - Western Blot Analysis of MCF-7 cells using E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/2208R).
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free [NBP3-07903]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free [NBP3-07903]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free [NBP3-07903] - Immunofluorescence Analysis of MCF-7 cells labeling CHD1 with E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/2208R) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free [NBP3-07903]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free [NBP3-07903]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free [NBP3-07903] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/2208R).
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Applications for E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2ug/million cells

Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

1-2ug/ml

Western Blot

1-2ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E-Cadherin Antibody (CDH1/2208R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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