DYKDDDDK Epitope Tag Antibody

Name: DYKDDDDK Epitope Tag Antibody
Brand: R&D Systems
SKU: MAB8529
Rating: 4 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB8529

Citations (3)

(2)

Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Immunogen:	DYKDDDDK epitope tag
Applications:	Flow Cytometry, Immunoprecipitation, Simple Western, Western Blot
Label:	Unconjugated
Source:	Recombinant Monoclonal Rabbit IgG Clone # 1042E
Specificity:	Detects N-terminal and C-terminal DYKDDDDK (FLAG^®) epitope tagged proteins in Western blot.

View Full Technical Details for DYKDDDDK Epitope Tag Antibody

Applications for DYKDDDDK Epitope Tag Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Simple Western	1 µg/mL	See below
Immunoprecipitation	1-4 µg/100 µg cell lysate	HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20, see our available Western blot detection antibodies.
Intracellular Staining by Flow Cytometry	0.001 µg/10⁶ cells	HEK293 cells transfected with FLAG, fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004).

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for DYKDDDDK Epitope Tag Antibody

	Western Blot Detection of DYKDDDDK (FLAG^®) Epitope Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1, N-terminal DYKDDDDK-tagged UCHL5, and N-terminal DYKDDDDK-tagged RNF20. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for DYKDDDDK-tagged proteins at approximately 55 kDa, 40 kDa, 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of DYKDDDDK (FLAG^®) Epitope Tag by Simple Western^TM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20, loaded at 0.1 mg/mL. Specific band were detected for DYKDDDDK-tagged proteins at approximately 64 and 136 kDa (as indicated) using 1 µg/mL of Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Intracellular Staining by Flow Cytometry Detection of DYKDDDDK Epitope Tag in HEK293 cells transfected with FLAG vs Irrelevant HEK293 wild type cells by Flow Cytometry. HEK293 cells transfected with DYKDDDDK-tagged proteins (filled histogram) vs Irrelevant HEK293 wild type cells (open histogram) were stained with Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529) at 0.001 ug/10⁶ cells followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Citations for DYKDDDDK Epitope Tag Antibody (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for DYKDDDDK Epitope Tag Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

WH Liu et al. (2021-01-01), A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models Scientific Reports, 11(1):12649.
PMID: 34135367
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

PR Kamble et al. (2021-01-01), N-glycosylation status of Trop2 impacts its surface density, interaction with claudin-7 and exosomal release Archives of biochemistry and biophysics, 714(0):109084.
PMID: 34774484
Species: Human
Sample Types: Cell Lystates
Applications: Immunoprecipitation

W Zhang et al. (2021-01-01), Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner Cell Death Disease, 2021;12(1):102.
PMID: 33473125

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Customer Reviews for DYKDDDDK Epitope Tag Antibody (2)

4 out of 5

2 customer ratings

5 stars

50%

4 stars

3 stars

50%

2 stars

1 star

Customer Images

Showing 1 - 2 of 2 reviews Showing All

Filter By:

DYKDDDDK Epitope Tag Antibody

Name: Anonymous

Application: WB

Sample Tested: HEK293 human embryonic kidney cell line

Species: Human

Verified Customer | Posted 09/01/2021
DYKDDDDK Epitope Tag Antibody

Name: Lucy Quach

Application: Simple Western

Sample Tested: FLAG tag protein,transduced cell lines

Species: Human

Verified Customer | Posted 11/02/2018

There are no reviews that match your criteria.

Showing 1 - 2 of 2 reviews Showing All

Product Documents for DYKDDDDK Epitope Tag Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

DYKDDDDK Epitope Tag Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for DYKDDDDK Epitope Tag Antibody

Scientific Data Examples for DYKDDDDK Epitope Tag Antibody

Western Blot

Simple Western

Intracellular Staining by Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Citations for DYKDDDDK Epitope Tag Antibody (3)

Customer Reviews for DYKDDDDK Epitope Tag Antibody (2)

Customer Images

DYKDDDDK Epitope Tag Antibody

DYKDDDDK Epitope Tag Antibody

Product Documents for DYKDDDDK Epitope Tag Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected