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Key Product Details

Species Reactivity

Drosophila

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant Drosophila Smad2
Gly262-Ser486
Accession # O96660

Specificity

Detects Drosophila Smad2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Smad1 is observed, and less than 1% cross-reactivity with rhSmad2, rhSmad3, and rhSmad5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Drosophila Smad2 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Smad2

Smad2 (SMAll body plus Mothers Against Decapentaplegic; also SmoX [Smad on Chr X]) is a 54 kDa (predicted) member of the receptor regulated Smad family of proteins. It is a downstream component of the Drosophila activin/TGF beta signaling pathway, and appears to play a key role in cell proliferation. Analogous to the system in vertebrates, stimulation of the fruitfly activin type I receptor (baboon):type II receptor (punt) complex results in dSmad2 (Drosophila Smad2) phosphorylation and subsequent interaction with dSmad4/Medea. As in vertebrates, the Drosophila Samd2/Smad4 complex enters the nucleus and interacts with a transcriptional cofactor(s) termed TGIF. At this point, the vertebrate:invertebrate systems diverge over the nature of the TGIF cofactor(s); vertebrate TGIF is a corepressor, while invertebrate TGIF (dTGIF) is a coactivator. Drosophila Smad2 is 486 amino acids (aa) in length. It contains two Mad homology domains (aa 7-130 and 285-475), the former of which participates in DNA-binding. Over aa 262-486, Drosophila Smad2 shares 70% aa sequence identity with both mouse and human Smad2.

Long Name

Mothers Against DPP Homolog 2

Alternate Names

JV18-1, MADH2, MADR2, Smox

Entrez Gene IDs

4087 (Human); 17126 (Mouse); 29357 (Rat); 31738 (Drosophila)

Gene Symbol

SMAD2

Additional Smad2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Drosophila Smad2 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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