DNMT1 Antibody (24GB5135)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51622
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB5135
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human DNMT1 (Uniprot: P26358)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DNMT1 Antibody (24GB5135)
Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody (24GB5135)[NBP3-51622] -
Immunocytochemical staining of HepG2 cells with DNMT1 antibody (1:1,000). Nuclei were stained blue with DAPI; DNMT1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Flow Cytometry: DNMT1 Antibody (24GB5135)[NBP3-51622] -
Validation of DNMT1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DNMT1 antibody (1:2,000) and analyzed using BD flow cytometer.
Flow Cytometry: DNMT1 Antibody (24GB5135)[NBP3-51622] -
Flow cytometric analysis of DNMT1expression in HepG2 cells using DNMT1 antibody (1:2,000). Green, isotype control; red, DNA methyltransferase 1.Applications for DNMT1 Antibody (24GB5135)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DNMT1
Improper methylation patterns have been linked to certain human tumors and developmental abnormalities. DNMT1 antibodies are useful tools for DNA methlyation research and studies on certian cancer.
Long Name
DNA [Cytosine-5-]-Methyltransferase 1
Alternate Names
CXXC9, MCMT
Gene Symbol
DNMT1
Additional DNMT1 Products
Product Documents for DNMT1 Antibody (24GB5135)
Product Specific Notices for DNMT1 Antibody (24GB5135)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...