DIAPH1 Antibody (6N10D0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15410

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15410-100ul
NBP3-15410-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6N10D0 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DIAPH1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DIAPH1 (O60610). MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQML

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DIAPH1 Antibody (6N10D0)

Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410]

Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410]

Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410] - Western blot analysis of extracts of 293T cells, using DIAPH1 Rabbit mAb (NBP3-15410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410]

Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410]

Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410] - Western blot analysis of extracts of various cell lines, using DIAPH1 Rabbit mAb (NBP3-15410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for DIAPH1 Antibody (6N10D0)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Western Blot

1:1000 - 1:3000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DIAPH1

Diaphanous 1 is a member of the Formin homology (FH) family of cytoskeletal organizer proteins. It is required for cytokinesis and transcriptional activation of the serum response factor. It has also been suggested to contribute to localized actin polymerization and stress fiber formation, both by recruiting profiling to sites of Rho action and by cooperating with ROCK. The human Diaphanous 1 gene is associated with Premature Ovarian Failure (POF) and Nonsyndromic Deafness (DFNA1), an autosomal dominant, fully penetrant progressive hearing loss.

Alternate Names

DIA1, diaphanous homolog 1 (Drosophila), hDIA1, homolog) 1, LFHL1FLJ25265

Gene Symbol

DIAPH1

Additional DIAPH1 Products

Product Documents for DIAPH1 Antibody (6N10D0)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DIAPH1 Antibody (6N10D0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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