DIAPH1 Antibody (23GB1990)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50967
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB1990
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human DIAPH1 (Uniprot: O60610)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DIAPH1 Antibody (23GB1990)
Immunocytochemistry/ Immunofluorescence: DIAPH1 Antibody (23GB1990)[NBP3-50967] -
Immunocytochemical staining of HepG2 cells with DIAPH1 antibody(1:1,000) . Nuclei were stained blue with DAPI; DIAPH1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: DIAPH1 Antibody (23GB1990)[NBP3-50967] -
Validation of DIAPH1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DIAPH1 antibody (1:2,000) and analyzed using BD flow cytometer.
Flow Cytometry: DIAPH1 Antibody (23GB1990)[NBP3-50967] -
Flow cytometric analysis of DIAPH1expression in HepG2 cells using DIAPH1 antibody (1:2,000). Green, isotype control; red, Diaphanous related formin 1.Applications for DIAPH1 Antibody (23GB1990)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DIAPH1
Alternate Names
DIA1, diaphanous homolog 1 (Drosophila), hDIA1, homolog) 1, LFHL1FLJ25265
Gene Symbol
DIAPH1
Additional DIAPH1 Products
Product Documents for DIAPH1 Antibody (23GB1990)
Product Specific Notices for DIAPH1 Antibody (23GB1990)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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