DHCR7 Antibody (HL3854)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48851
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL3854
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide of human DHCR7.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DHCR7 Antibody (HL3854)
Immunohistochemistry-Paraffin: DHCR7 Antibody (HL3854) [NBP3-48851] -
DHCR7 antibody [HL3854] detects DHCR7 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.
DHCR7 stained by DHCR7 antibody [HL3854] (NBP3-48851) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
Western Blot: DHCR7 Antibody (HL3854) [NBP3-48851] -
Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL3854] (NBP3-48851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: DHCR7 Antibody (HL3854) [NBP3-48851] -
Boiled and unboiled MCF-7 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL3854] (NBP3-48851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibodyApplications for DHCR7 Antibody (HL3854)
Application
Recommended Usage
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DHCR7
Alternate Names
7-dehydrocholesterol reductase, 7-DHC reductase, D7SR, delta-7-dehydrocholesterol reductase, EC 1.3.1, EC 1.3.1.21, Putative sterol reductase SR-2, SLOS, Smith-Lemli-Opitz syndrome, Sterol Delta(7)-reductase, sterol delta-7-reductase
Gene Symbol
DHCR7
Additional DHCR7 Products
Product Documents for DHCR7 Antibody (HL3854)
Product Specific Notices for DHCR7 Antibody (HL3854)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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