Defensin alpha 5 Antibody (8C8) [PerCP]
Novus Biologicals, part of Bio-Techne | Catalog # NB110-60002PCP
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Dot Blot
Label
PerCP (Excitation = 490 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 8C8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Full-length human Defensin alpha 5 [Swiss-Prot# Q01523]. The propeptide is the full-length protein. In the mature form, it is cleaved at residue 63 (alanine).
Reactivity Notes
Human.
Localization
Secreted protein.
Specificity
This is specific for HD5. It does not cross-react with HD6, lysozyme or sPLA2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Defensin alpha 5 Antibody (8C8) [PerCP]
Application
Recommended Usage
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Defensin alpha 5 antibody is useful for Immunohistochemistry paraffin embedded sections, Western blot and Immunocytochemistry/Immunofluorescence. In Western blot a band is observed ~10 kDa. Dot Blot and ELISA were reported in scientific literature.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: alpha-Defensin 5
Defensin alpha 5 antibodies are useful tools for bacterial defense studies and human immunology research.
Long Name
Defensin Alpha 5
Alternate Names
DEF5, DEFA5, Defensin alpha 5, HD-5
Gene Symbol
DEFA5
Additional alpha-Defensin 5 Products
Product Documents for Defensin alpha 5 Antibody (8C8) [PerCP]
Product Specific Notices for Defensin alpha 5 Antibody (8C8) [PerCP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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