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Cytokeratin 19 Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-687F

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NB100-687F
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Orangutan (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide derived from the C-terminal region of human Cytokeratin 19 (between residues 350-400) [UniProt P08727]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25401473)

Localization

Cytoplasmic.

Marker

Epithelial Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for Cytokeratin 19 Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB100-687. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cytokeratin 19

Cytokeratin 19 (KRT19) is an intermediate filament (IF) protein that is part of the Keratin family of proteins. This protein is also known by the names of Keratin 19 or Keratin type I Cytoskeletal 19. The Keratin family consists of Keratin 1-20, which are divided into two subtypes: Type 1 contains the acidic Cytokeratin proteins consisting of Cytokeratins 9-20, including KRT19; Type 2 Keratins include the basic to neutral protein and contains Cytokeratin 1-8. Type 1 and Type 2 Keratins often form oligomers, interacting to create heterotypic chains. However, KRT19 is unique in that while being the smallest of the Type 1 Keratin proteins, it does not interact with any Type 2 Keratins. IF proteins such as KRT19 are integral for defining and maintaining cellular structure and morphology, and are important for cellular integrity by anchoring to membrane complexes via proteins such as Epiplakin, Trichoplein, Periplakin, Plakophilins, Plectin, BPAG1, Desmoplakin, and Envoplakin. KRT19 is also involved in the organization of the myofibers of striated muscle. KRT19 and other Cytokeratin proteins are mainly found in epithelial cells, with KRT19 being expressed exclusively in defined zones of basal keratinocytes. KRT19 is an important biomarker for breast cancer cells, tumor cells of the lymph nodes, peripheral blood, and bone marrow. Cytokeratin 19 can be expressed with KRT8 and KRT18 to differentiate cells of epithelial lines from hematopoietic tumor cells in the peripheral blood.

Alternate Names

CK19, EndoC, K19, Krt19

Gene Symbol

KRT19

Additional Cytokeratin 19 Products

Product Documents for Cytokeratin 19 Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cytokeratin 19 Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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