Cytokeratin 19 Antibody - BSA Free

Immunohistochemistry: Cytokeratin 19 Antibody - BSA Free [NB100-687]

Cytokeratin-19-Antibody-Immunohistochemistry-NB100-687-img0012.jpg

Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody - BSA Free [NB100-687]

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in MCF7 cells using Cytokeratin 19 antibody (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Flow Cytometry: Cytokeratin 19 Antibody - BSA Free [NB100-687]

Flow Cytometry: Cytokeratin 19 Antibody [NB100-687] - An intracellular stain was performed on MCF7 cells with Cytokeratin 19 Antibody NB100-687AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Western Blot: Cytokeratin 19 AntibodyBSA Free [NB100-687]

Western Blot: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in 1) HepG2 and 2) MCF7 lysates.

Immunohistochemistry: Cytokeratin 19 Antibody - BSA Free [NB100-687]

Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Staining of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.

Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody - BSA Free [NB100-687] -

Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody - BSA Free [NB100-687] - TUDCA induces intermediate states of mesenchymal & epithelial cells in primary cultures of rat HSC during hepatic differentiation.Freshly isolated rat HSC (a) exhibited typical lipid droplets that contained retinoids & (b) expressed the mesenchymal filament proteins vimentin (red) & desmin (green) as investigated by immunofluorescence. (c) Under control conditions HSC remained negative for K19 expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 μM TUDCA for 14 days (red). (e–g) K19 was found to be co-expressed with desmin & vimentin (green), which indicated the origin of epithelial progenitor cells from HSC. (h–k) Also K18, Afp & Mrp2 (red), which served as markers for hepatic differentiation, were co-expressed with vimentin & desmin (green) after 21 days of TUDCA treatment. Cells with different states of maturation were still found after 21 days of TUDCA treatment. Some cells developed into hepatocyte-like cells with (j) dominant K18 filaments (white arrows) while others remained immature with persisting desmin (red arrow) or (l) K19 protein residues. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep13320), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Cytokeratin 19 in MCF7 Human Cell Line.

Cytokeratin 19 was detected in immersion fixed MCF7 human breast cancer cell line using Rabbit anti-Cytokeratin 19 Antigen Affinity Purified Polyclonal Antibody conjugated to Biotin (Catalog # NB100-687B) at 5 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.