csl/RBPJK Antibody - BSA Free

Western Blot: csl/RBPJK Antibody [NBP1-33427] -

Western Blot: csl/RBPJK Antibody [NBP1-33427] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with csl/RBPJK antibody [C1C3] (NBP1-33427) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody,

Immunocytochemistry/ Immunofluorescence: csl/RBPJK Antibody [NBP1-33427]

Immunocytochemistry/Immunofluorescence: csl/RBPJK Antibody [NBP1-33427] - Analysis of paraformaldehyde-fixed HeLa at 1:100 dilution.

Chromatin Immunoprecipitation (ChIP): csl/RBPJK Antibody [NBP1-33427] - Sheared, cross-linked chromatin was prepared from Jurkat T-ALL cells using the Covaris TruChIP kit and shearing with the LE220 sonicator. The chromatin buffer was adjusted to 150 mM NaCl, 20 mM Tris-Cl pH 8, 2 mM EDTA, and 1% Triton X-100. Three antibodies, as well as pure pre-immune rabbit IgG control, were added to each IP reaction, consisting of 7 million cells, and incubated overnight with mixing at 4 C. The antibodies were captured with Magna ChIP Protein A+G beads (Millipore), washed with three wash buffers, and the DNA eluted and purified with the iPure v2 kit (Diagenode). The purified DNA was normalized to the input control with qPCR quantification of the insulin exon 1 gene (INS) and an RBPJ binding site in the promoter of HES1. Image from verified customer review.