CS Citrate Synthase Antibody (CL2561) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44452

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2561

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CS Citrate Synthase Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O75390, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for CS Citrate Synthase Antibody (CL2561) - Azide and BSA Free

CS Citrate Synthase Antibody (CL2561) - Azide and BSA Free Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP3-44452]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP3-44452]

Analysis in human heart muscle and liver tissues using NBP3-44452 antibody. Corresponding CS Citrate Synthase RNA-seq data are presented for the same tissues.
CS Citrate Synthase Antibody (CL2561) - Azide and BSA Free Western Blot: CS Citrate Synthase Antibody [NBP3-44452]

Western Blot: CS Citrate Synthase Antibody [NBP3-44452]

Lane 1: Marker [kDa]
Lane 2: Human cell line U-251 MG
CS Citrate Synthase Antibody (CL2561) - Azide and BSA Free Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP3-44452]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP3-44452]

Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
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Applications for CS Citrate Synthase Antibody (CL2561) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CS Citrate Synthase

The protein encoded by the CS Citrate Synthase gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citratefrom oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. Thisprotein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. (provided byRefSeq)

Long Name

CS Citrate Synthase

Alternate Names

citrate (Si)-synthase, citrate synthase, mitochondrial, EC 2.3.3.1

Gene Symbol

CS

Additional CS Citrate Synthase Products

Product Documents for CS Citrate Synthase Antibody (CL2561) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CS Citrate Synthase Antibody (CL2561) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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