Detects CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human RANTES and recombinant mouse RANTES is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Cotton Rat CCL5/RANTES Antibody

Chemotaxis Induced by CCL5/RANTES and Neutralization by Cotton Rat CCL5/RANTES Antibody.

Recombinant Cotton Rat CCL5/RANTES (Catalog # 1010-CR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL5/RANTES (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1010). The ND₅₀ is typically 1-4 µg/mL.

Applications for Cotton Rat CCL5/RANTES Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Cotton Rat CCL5/RANTES (Catalog # 1010-CR)

Neutralization

Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CCR5. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 0.2 µg/mL Recombinant Cotton Rat CCL5/RANTES.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta‑chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1‑3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature cotton rat CCL5 shares 88% aa seqeuence identity with mouse and rat CCL5 and 71%‑77% with canine, feline, and human CCL5. CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (5, 6). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (7). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (8). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (9). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (10‑12). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (13‑15). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (16).

References

Schall, T.J. et al. (1990) Nature 347:669.
Bacon, K.B. et al. (1995) Science 269:1727.
Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
Levy, J.A. (2009) J. Immunol. 182:3945.
Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401.
Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. 100:1885.
von Hundelshausen, P. et al. (2005) Blood 105:924.
von Hundelshausen, P. et al. (2001) Circulation 103:1772.
Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
Baltus, T. et al. (2003) Blood 102:1985.
Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.

Alternate Names

RANTES, SISd

Entrez Gene IDs

6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)

Gene Symbol

CCL5

UniProt

AAL16932

Additional CCL5/RANTES Products

Product Documents for Cotton Rat CCL5/RANTES Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Cotton Rat CCL5/RANTES Antibody

For research use only

Citations
Reviews

Cotton Rat CCL5/RANTES Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1010

Key Product Details

Species Reactivity

Validated:

Cited:

Applications