Recombinant Cotton Rat CCL5/RANTES Protein

Name: Recombinant Cotton Rat CCL5/RANTES Protein
Brand: R&D Systems
SKU: 1010-CR
Price: 340 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1010-CR

Citations (3)

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Key Product Details

Source:	E. coli
Accession #:	AAL16932
Applications:	Bioactivity

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Product Specifications for Recombinant Cotton Rat CCL5/RANTES Protein

Source	E. coli-derived cotton rat CCL5/RANTES protein Ser24-Lys91
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser24
Predicted Molecular Mass	7.9 kDa
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR5. The ED₅₀ for this effect is 20-100 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1010-CR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1010-CR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1-3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature cotton rat CCL5 shares 88% aa seqeuence identity with mouse and rat CCL5 and 71%-77% with canine, feline, and human CCL5. CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (5, 6). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (7). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (8). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (9). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (10-12). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (13-15). CCL5 is up-regulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (16).

Item	Value
References	Schall, T.J. et al. (1990) Nature 347:669. Bacon, K.B. et al. (1995) Science 269:1727. Fischer, F.R. et al. (2001) J. Immunol. 167:1637. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83. Levy, J.A. (2009) J. Immunol. 182:3945. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37. DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401. Proost, P. et al. (1998) J. Biol. Chem. 273:7222. Appay, V. et al. (1999) J. Biol. Chem. 274:27505. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. 100:1885. von Hundelshausen, P. et al. (2005) Blood 105:924. von Hundelshausen, P. et al. (2001) Circulation 103:1772. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897. Baltus, T. et al. (2003) Blood 102:1985. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.
Entrez Gene IDs	6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names	CCL5, D17S136Enormally T-expressed, and presumably secreted, EoCP, Eosinophil chemotactic cytokine, RANTES, SIS-delta, SISd, Small-inducible cytokine A5, T cell-specific protein P228, T-cell specific protein p288, TCP228T-cell-specific protein RANTES, chemokine (C-C motif) ligand 5, small inducible cytokine A5 (RANTES), small inducible cytokine subfamily A (Cys-Cys), member 5

Citations for Recombinant Cotton Rat CCL5/RANTES Protein (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Cotton Rat CCL5/RANTES Protein include:

Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Nathalie Strazielle et al. (2016), T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium. PLoS ONE, 0(0):1932-6203.
PMID: 26942913
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Kim H et al. (2014), CCL5 upregulates activation of AMP-activated protein kinases in vascular smooth muscle cells of spontaneously hypertensive rats. Cytokine, 67(2):77-84.
PMID: 24656927
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Benamar K et al. (2008), Elevated level of the proinflammatory chemokine, RANTES/CCL5, in the periaqueductal grey causes hyperalgesia in rats. Eur. J. Pharmacol., 592(1):93-5.
PMID: 18656466

Recombinant Cotton Rat CCL5/RANTES Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Cotton Rat CCL5/RANTES Protein

Formulation, Preparation and Storage

Carrier Free

1010-CR

Carrier

1010-CR/CF

Carrier Free

Reconstitution Calculator

Background: CCL5/RANTES

Citations for Recombinant Cotton Rat CCL5/RANTES Protein (3)

Product Documents for Recombinant Cotton Rat CCL5/RANTES Protein

Product Datasheets

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SDS

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