Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (HCGa/2728R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14273
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # HCGa/2728R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa1-100) of human Chorionic Gonadotropin alpha Chain (hCG alpha) protein (exact sequence is proprietary) (Uniprot: P01215)
Localization
Cytoplasmic, secreted
Marker
Pregnancy & Choriocarcinoma Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13844).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images
Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (HCGa/2728R)
Formalin-fixed, paraffin-embedded human placenta stained with Chorionic Gonadotropin alpha Chain (hCG alpha) antibody (HCGa/2728R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (HCGa/2728R)
Formalin-fixed, paraffin-embedded human placenta stained with Chorionic Gonadotropin alpha Chain (hCG alpha) antibody (HCGa/2728R). Inset: PBS instead of primary antibody; secondary only negative control.Applications
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Chorionic Gonadotropin alpha Chain (HCG alpha)
HCG is a glycoprotein hormone secreted in pregnancy by embryonic trophoblast cells during the first 7 weeks of gestation. Production subsequently shifts to the placenta, where it continues until term. HCG is a heterodimer consisting of an alpha chain and a beta chain. The alpha chain is common to several other hormones, including lutenizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the beta chain is unique to HCG. Both alpha and beta chains contain a cysteine knot motif, also found in growth factors such as TGF-beta family members.
Alternate Names
alpha HCG, CGA, FSHA, GPHa1, LHA, TSHA
Gene Symbol
CGA
Additional Chorionic Gonadotropin alpha Chain (HCG alpha) Products
- All Products for Chorionic Gonadotropin alpha Chain (HCG alpha)
- Chorionic Gonadotropin alpha Chain (HCG alpha) ELISA Kits
- Chorionic Gonadotropin alpha Chain (HCG alpha) Lysates
- Chorionic Gonadotropin alpha Chain (HCG alpha) Primary Antibodies
- Chorionic Gonadotropin alpha Chain (HCG alpha) Proteins and Enzymes
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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