CFTR Antibody (CFTR/1643)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53375
![Immunohistochemistry-Paraffin: CFTR Antibody (CFTR/1643) [NBP2-53375]](https://resources.bio-techne.com/images/products/CFTR-Antibody-CFTR-1643-Immunohistochemistry-Paraffin-NBP2-53375-img0001.jpg "Immunohistochemistry-Paraffin: CFTR Antibody (CFTR/1643) [NBP2-53375]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # CFTR/1643
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 258-385) of human CFTR protein (exact sequence is proprietary) (Uniprot: P13569)
Localization
Cell Surface and Cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54509)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for CFTR Antibody (CFTR/1643)
Immunohistochemistry-Paraffin: CFTR Antibody (CFTR/1643) [NBP2-53375]
Immunohistochemistry-Paraffin: CFTR Antibody (CFTR/1643) [NBP2-53375] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CFTR Monoclonal Antibody (CFTR/1643).![Flow Cytometry: CFTR Antibody (CFTR/1643) [NBP2-53375]](https://resources.bio-techne.com/images/products/CFTR-Antibody-CFTR-1643-Flow-Cytometry-NBP2-53375-img0002.jpg "Flow Cytometry: CFTR Antibody (CFTR/1643) [NBP2-53375]")
Flow Cytometry: CFTR Antibody (CFTR/1643) [NBP2-53375]
Flow Cytometry: CFTR Antibody (CFTR/1643) [NBP2-53375] - Analysis of MOLT-4 cells using CFTR Antibody (CFTR/1643) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).![Protein Array: CFTR Antibody (CFTR/1643) [NBP2-53375]](https://resources.bio-techne.com/images/products/CFTR-Antibody-CFTR-1643-Protein-Array-NBP2-53375-img0003.jpg "Protein Array: CFTR Antibody (CFTR/1643) [NBP2-53375]")
Protein Array: CFTR Antibody (CFTR/1643) [NBP2-53375]
Protein Array: CFTR Antibody (CFTR/1643) [NBP2-53375] - Analysis of Protein Array containing >19,000 full-length human proteins using CFTR Antibody (CFTR/1643) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.Applications for CFTR Antibody (CFTR/1643)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CFTR
Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP-binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).
Long Name
Cystic Fibrosis Transmembrane Conductance Regulator
Alternate Names
ABC35, ABCC7CF, ATP-binding cassette sub-family C member 7, ATP-binding cassette transporter sub-family C member 7, cAMP-dependent chloride channel, CFTR/MRP, Channel conductance-controlling ATPase, cystic fibrosis transmembrane conductance regulator, cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7), cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7), dJ760C5.1, EC 3.6.3, MRP7EC 3.6.3.49, TNR-CFTR
Gene Symbol
CFTR
UniProt
Additional CFTR Products
Product Documents for CFTR Antibody (CFTR/1643)
Product Specific Notices for CFTR Antibody (CFTR/1643)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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