CD68/SR-D1 Antibody (KP1) [DyLight 550]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-683R
Clone KP1 was used by HLDA to establish CD designation.
![CD68/SR-D1 Antibody (KP1) [DyLight 550]](https://resources.bio-techne.com/images/products/nb100-683r_mouse-monoclonal-cd68-sr-d1-antibody-kp1-dylight-550-immunocytochemistry-immunofluorescence-2792024135243..jpg "CD68/SR-D1 (KP1) in A431 Human Cell Line.")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Electron Microscopy
Label
DyLight 550 (Excitation = 562 nm, Emission = 576 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # KP1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This CD68/SR-D1 Antibody (KP1) was developed against subcellular fraction of human alveolar macrophages.
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 25058444)
Specificity
This CD68/SR-D1 Antibody (KP1) is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for CD68/SR-D1 Antibody (KP1) [DyLight 550]
CD68/SR-D1 (KP1) in A431 Human Cell Line.
CD68/SR-D1 (KP1) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti-CD68/SR-D1 (KP1) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NB100-683R) (red) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.![CD68/SR-D1 Antibody (KP1) [DyLight 550]](https://resources.bio-techne.com/images/products/nb100-683r_mouse-monoclonal-cd68-sr-d1-antibody-kp1-dylight-550-flow-intracellular--3092024154559..jpg "Detection of CD68/SR-D1 (KP1) in A431 Human Cell Line by Flow Cytometry.")
Detection of CD68/SR-D1 (KP1) in A431 Human Cell Line by Flow Cytometry.
An intracellular stain was performed on A431 human skin carcinoma cell line using Mouse anti- CD68/SR-D1 (KP1) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NB100-683R, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.Applications for CD68/SR-D1 Antibody (KP1) [DyLight 550]
Application
Recommended Usage
Dual RNAscope ISH-IHC
Optimal dilutions of this antibody should be experimentally determined.
Electron Microscopy
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB100-683. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CD68/SR-D1
CD68 is highly expressed in cells of the mononuclear phagocyte system such as macrophages, microglia, osteoclasts, and myeloid dendritic cells (DCs); and is expressed to a lesser extent in lymphoid cells (CD19+ B lymphocytes and CD4+ T lymphocytes), human umbilical cord mesenchymal stem cells (MSCs), fibroblasts, endothelial cells, multiple non-hematopoietic cancer cell lines, and human arterial intimal smooth muscle cells (SMCs). Expression has been also observed in diseased states for granulocytes and neutrophils, in particular basophils from myeloproliferative disorders and intestinal neutrophils from inflammatory bowel disease (IBD), respectively (1).
Although the function of CD68 has yet to be established, it has often been used as an immunohistochemistry (IHC) marker of inflammation and for granular cell tumors (GCTs). CD68+ tumor associated macrophages (TAMs) has been suggested to be a predictive marker for poor cancer prognosis, but a meta-analysis showed the presence of CD68 is not correlated with survival (2). In addition, a role in hepatic malaria infection has been reported based on the finding that peptide P39 binds CD68, considered a receptor for malaria sporozoite, and inhibits parasite entry into Kupffer cells. CD68 was deemed a member of the Scavenger receptor family due to its upregulation in macrophages following inflammatory stimuli, ability to bind modified LDL, phosphatidylserine, and apoptotic cells, as well as shuttling between the plasma membrane and endosomes. CD68 has been linked to atherogenesis based on binding and internalization of its ligand, oxLDL (1).
References
1. Chistiakov, DA, Killingsworth, MC, Myasoedova, VA. Orekhov AN, Bobryshev YV. (2017) CD68/macrosialin: not just a histochemical marker. Lab Invest. 97:4-13. PMID: 27869795
2. Troiano G, Caponio VCA, Adipietro I, Tepedino M, Santoro R, Laino L, Lo Russo L, Cirillo N, Lo Muzio L. (2019) Prognostic significance of CD68+ and CD163+ tumor associated macrophages in head and neck squamous cell carcinoma: A systematic review and meta-analysis. Oral Oncol. 93:66-75. PMID: 31109698.
Alternate Names
CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1
Gene Symbol
CD68
Additional CD68/SR-D1 Products
Product Documents for CD68/SR-D1 Antibody (KP1) [DyLight 550]
Product Specific Notices for CD68/SR-D1 Antibody (KP1) [DyLight 550]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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