CD3 zeta [p Tyr111] Antibody (EM-55) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37709CL1
![CD3 zeta [p Tyr111] Antibody (EM-55) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/nbp2-37709cl1_mouse-cd3-zeta-p-tyr111-mab-em-55-corafluor-1-31122025134332.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Flow Cytometry, Flow (Intracellular)
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # EM-55
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
BP1 (pTyr111/123) - KLH.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Modification
p Tyr111
Specificity
Antibody recognizes phosphorylated tyrosine 111 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for CD3 zeta [p Tyr111] Antibody (EM-55) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for CD3 zeta [p Tyr111] Antibody (EM-55) [CoraFluor™ 1]
Application
Recommended Usage
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-37709. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CD3 zeta/CD247
Long Name
T-cell Receptor zeta
Alternate Names
CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain
Gene Symbol
CD247
Additional CD3 zeta/CD247 Products
Product Documents for CD3 zeta [p Tyr111] Antibody (EM-55) [CoraFluor™ 1]
Product Specific Notices for CD3 zeta [p Tyr111] Antibody (EM-55) [CoraFluor™ 1]
Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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