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CD161 Antibody (10/78) - BSA Free, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB100-65297

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NB100-65297
NB100-65297SS

Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10/78

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for CD161 Antibody (10/78) - BSA Free

Immunogen

Purified splenic NK cells from the LEW rat strain.

Specificity

Recognizes the rat CD161 protein (also known as NKR-PI) a 60kD glycoprotein expressed on rat NK cells and T cell subpopulations. This competes with clone 3.2.3 for binding to antigen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD161 Antibody (10/78) - BSA Free

Flow Cytometry: CD161 Antibody (10/78) [NB100-65297] - Rat splenocytes were stained with Anti-Rat CD3 APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat CD161 Monoclonal Antibody (Catalog# NB100-65297) or (B) Mouse IgG1 Isotype control (Catalog# MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog# F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for CD161 Antibody (10/78) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NB100-65297 in the following applications:

Published Applications

Read 1 publication using NB100-65297 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD161

CD161, also known as KLRB1, NK1.1, and NKR-P1A is a 40 kDa type II transmembrane glycoprotein that contains one C-type lectin domain in its extracellular region. CD161 is expressed as a disulfide-linked dimer on the surface of Th17 cells and NK cells as well as on subsets of CD1-restricted T cells, intestinal NT cells, peripheral memory T cells, monocytes, and dendritic cells. It binds to OCIL/CLEC2d, leading to an inhibition of NK cell-mediated cytotoxicity and IFN-gamma secretion. Alternatively, CD161 can enhance TCR activation to CD1d ligation. CD161+ cell populations are depleted in ulcerative colitis, Grave's disease, and AIDS, although CD161+ T cells are activated during asthmatic attacks. Additional related proteins are expressed in mouse but not human: the inhibitory NKR-P1B and D, and the stimulatory NKR-P1A, C, and F.

Alternate Names

CD161, CLEC5B, KLRB1, Ly59, NKR, NKRP1, NKRP1A

Entrez Gene IDs

683758 (Rat)

Gene Symbol

KLRB1

UniProt

Additional CD161 Products

Product Documents for CD161 Antibody (10/78) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD161 Antibody (10/78) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for CD161 Antibody (10/78) - BSA Free

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  • Q: I see that this antibody is listed for use in IHC...would that include IHC on formalin-fixed-paraffin-embedded tissue?

    A: NB100-65297 has been tested and validated for use with frozen tissues however its performance has not been evaluated in paraffin embedded tissues yet and therefore cannot be guaranteed. If you would be interested in testing this antibody in IHC-Paraffin please take a look at our Innovators Reward Program.

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