CD117/c-kit Antibody (CL1657) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57842

Recombinant Monoclonal Antibody
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NBP3-57842-100ul
NBP3-57842-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL1657

Format

BSA Free

Concentration

1 mg/ml

View all CD117/c-kit Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P10721, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD117/c-kit Antibody (CL1657) - BSA Free

CD117/c-kit Antibody (CL1657) - BSA Free

CD117/c-kit Antibody (CL1657) - BSA Free

Analysis in human cell line RT-4.
CD117/c-kit Antibody (CL1657) - BSA Free

CD117/c-kit Antibody (CL1657) - BSA Free

Lane 1: Marker [kDa]
Lane 2: Human cell line RT-4
CD117/c-kit Antibody (CL1657) - BSA Free

CD117/c-kit Antibody (CL1657) - BSA Free

Staining of human cerebellum shows moderate positivity in neuropil of the molecular layer.
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Applications for CD117/c-kit Antibody (CL1657) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2), 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (CL1657) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD117/c-kit Antibody (CL1657) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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