Key Product Details

Species Reactivity

Mouse

Applications

ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), Neutralization

Label

mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 158132

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CCL22/MDC Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL22/MDC
Gly25-Ser92
Accession # O88430

Specificity

Detects mouse CCL22/MDC in ELISAs and Western blots. In Western blots, this antibody shows 25% cross-reactivity with recombinant viral MIP‑II and no cross-reactivity with rmCCL1, 2, 3, 4, 6, 7, CCL9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 24, 25, 27, rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, or rrCCL20.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for CCL22/MDC Antibody (158132) [mFluor Violet 500 SE]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB4391. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CCL22/MDC

Chemokines are a large family of small secreted proteins that regulate migration of white blood cells. Based on the arrangement of the first two of the four conserved cysteine residues, chemokines are classified into four subfamilies, CXC, CC, C and recently identified CX3C (1). The CC chemokines, in which the first 2 cysteines are adjacent, usually act on monocytes, T lymphocytes, and, in some cases, eosinophils, basophils, or mast cells. The CC subfamily proteins are 70 to 100 amino acids long, have 25 to 75% identity with each other, and include novel member of the CC chemokine subfamily, designated ABCD1 (SCYA22). The SCYA22 protein shares 28 to 34% identity with other CC chemokines and contains the characteristic 4-cysteine motif and 9 other highly conserved residues (2). The activated T cell-attracting CC chemokine CCL22 is expressed by stimulated B cells and mature dendritic cells (DC). Both identical and distinct proteins contribute to expression of CCL22 in DC and B cells (3).

Alternate Names

ABCD-1, MDC, STCP-1

Gene Symbol

CCL22

Additional CCL22/MDC Products

Product Documents for CCL22/MDC Antibody (158132) [mFluor Violet 500 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CCL22/MDC Antibody (158132) [mFluor Violet 500 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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