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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # CPP32 4-1-18

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free

Immunogen

This Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free was developed against full-length recombinant human Caspase 3 [UniProt# P42574].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free

Western Blot: Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free [NBP2-80614] - Detection of Caspase (19 and 35 kDa) from HEK293 cell extract using Caspase-3 Antibody (CPP32 4-1-18) (NB500-210). Lanes 1 and 2 contain inactive and active Caspase, respectively. Image from the standard format of this antibody.
Immunocytochemistry: Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free [NBP2-80614] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder 1:300 dilution of mouse monoclonal Caspase-3 Antibody (CPP32 4-1-18) (NB500-210, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte H
Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free [NBP2-80614] - Rat epithelial cells of the tongue base. Antigen retrieval method: Citrate buffer. Image from the standard format of this antibody.

Applications for Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

: 2 ug/ml

Immunohistochemistry

: 1:200-1:500

Immunohistochemistry-Frozen

reported in scientific literature (PMID 31242448)

Immunohistochemistry-Paraffin

: 1:200-1:500

Immunoprecipitation

: 2 ug / mg lysate

Simple Western

: 1:2000

Western Blot

: 1:500-1:1000
Application Notes
A band is seen at ~32 kDa for the inactive form of Caspase 3 and ~17-22 kDa for the active form of Caspase 3 in Western Blot. (See protocol for additional information).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-3

CPP32 encodes a member of the cysteine-aspartic acid protease (caspase) family that plays a key role in apoptosis. Caspase 3 (also known as CPP32, Apopain, Yama, and PARP cleavage protease) is the most extensively studied apoptotic protein among caspase family members (1-2). Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). Alternative splicing of CPP32 results in two transcript variants which encode the same protein. The processed form of Caspase 3 consists of large (theoretical molecular weight 17kD) and small (theoretical molecular weight 12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease (3).

References

1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G., . . . Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3

2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y., . . . Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199

3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A., . . . Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434

Alternate Names

Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA

Gene Symbol

CASP3

Additional Caspase-3 Products

Product Documents for Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Caspase-3 Antibody (CPP32 4-1-18) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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