Caspase-2 Antibody (23GB3305)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51211
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB3305
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Caspase-2 (Uniprot: P42575)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Caspase-2 Antibody (23GB3305)
Immunocytochemistry/ Immunofluorescence: Caspase-2 Antibody (23GB3305)[NBP3-51211] -
Immunocytochemical staining of HepG2 cells with Caspase-2 antibody (1:1,000). Nuclei were stained blue with DAPI; Caspase 2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Western Blot: Caspase-2 Antibody (23GB3305)[NBP3-51211] -
Western blotting analysis using Caspase-2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for Caspase-2 Antibody (23GB3305)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Caspase-2
Caspase-2, also known as NEDD2 and ICH-1, is a 30‑32 kDa member of the peptidase C14A/IL‑1 beta‑converting family of enzymes. It is widely expressed and is an integral component of the apoptotic cascade. Caspase-2 is a disulfide-linked homodimer, with activation occurring following oligomerization within the context of activating platforms such as DISC (death-inducing signaling complex) or the PIDDosome. Initially, Pro-Caspase-2 is cleaved into a p34 subunit and a p14 subunit which are further processed to generate the prodomain, plus the mature p18 and p12 subunits. Notably, each p18-p12 heterodimer demonstrates proteolytic activity and localizes to either the nucleus or cytoplasm.
Alternate Names
CASP2, Caspase2, ICH-1, NEDD2
Gene Symbol
CASP2
Additional Caspase-2 Products
Product Documents for Caspase-2 Antibody (23GB3305)
Product Specific Notices for Caspase-2 Antibody (23GB3305)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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