Caspase-11/Caspase-4 Antibody (1172C) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB8648CL1
Recombinant Monoclonal Antibody
![Caspase-11/Caspase-4 Antibody (1172C) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/fab8648cl1_rabbit-caspase-11-caspase-4-mab-1172c-corafluor-1-12920251222117.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
CoraFluor 1
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1172C
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with mouse Caspase-11/Caspase-4
Accession # P70343
Accession # P70343
Specificity
Detects mouse Caspase-11/Caspase-4 in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for Caspase-11/Caspase-4 Antibody (1172C) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Caspase-11/Caspase-4 Antibody (1172C) [CoraFluor™ 1]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB8648. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Caspase-11/Caspase-4
Caspase-11 (also known as as Caspase-4) is a cysteine-aspartic acid protease that functions in apoptosis initiation and the activation of proinflammatory cytokines. Pro-Caspase-11 is expressed as a zymogen which is cleaved into two subunits, p20 and p10. It is activated by an autocatalytic mechanism or by Caspase-8 mediated cleavage in response to ER stress or bacterial infection. Its expression and activation can be induced by exposure to LPS. Caspase-11 is one of several caspases associated with the inflammasome and plays an important role in the activation of proinflammatory cytokines. The active enzyme can cleave and activate Caspase-1 which in turn activates IL-1 beta.
Alternate Names
CASP11, Caspl, Ich-3
Gene Symbol
Casp4
Additional Caspase-11/Caspase-4 Products
Product Documents for Caspase-11/Caspase-4 Antibody (1172C) [CoraFluor™ 1]
Product Specific Notices for Caspase-11/Caspase-4 Antibody (1172C) [CoraFluor™ 1]
Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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