Bmf Antibody - BSA Free

Western Blot: Bmf AntibodyBSA Free [NBP1-76658]

Bmf-Antibody-Western-Blot-NBP1-76658-img0009.jpg

Western Blot: Bmf AntibodyBSA Free [NBP1-76658]

Western Blot: Bmf Antibody [NBP1-76658] - Analysis of Bmf in 293 cell lysate with Bmf antibody at (A) 1 and (B) 2 ug/ml.

Western Blot: Bmf Antibody - BSA Free [NBP1-76658] -

Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs incubated w/ M-CSF (30 ng/ml) & RANKL (40 ng/ml) for 40 h, washed thoroughly, & incubated further w/ LPS (50 ng/ml) in presence of M-CSF (30 ng/ml) for 48 h. (a) Cells transfected w/ 30 nM of miR-29b mimic or con mimic in presence of M-CSF (30 ng/ml) for 6 h. Total RNA analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, & HRK. Expression levels w/ con mimic treatment set at 1. Cell lysates subjected to WB analysis w/ antibodies against BMF, PUMA, BAK1, BIM, HRK, & BCL-2. Antibodies against beta-actin used for normalization. (b) w/out transfection, total RNA analyzed by qPCR to quantify the expression of BMF, & cell lysates subjected to WB analysis w/ anti-BMF Ab. (c) Total RNA & tissue lysate of tibiae from LPS-treated or vehicle-treated (V, PBS) mice analyzed by qPCR to quantify the expression of BMF & subjected to WB analysis w/ antibodies against BMF. (d) Cells thoroughly washed, transfected w/ 30 nM of anti-miR-29b or con inh, & stimulated w/ LPS (50 ng/ml) in presence of M-CSF. After 48 h, total RNA analyzed by qPCR to quantify BMF expression. Expression levels w/ con inh treatment set at 1.0. Cell lysates subjected to WB analysis w/ anti-BMF & anti-BCL-2 Ab. (e) Cells thoroughly washed, transfected w/ 50 nM of scRNA or siBMF, stimulated w/ LPS (50 ng/ml) in presence of M-CSF for 48 h, & analyzed to measure TRAP-positive MNCs & annexin V-positive cells. siRNA-mediated silencing of BMF confirmed by RT-PCR & qPCR. The Ct values of genes widely distributed between 17.33 & 30.92. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 compared w/ each corresponding control. Similar results obtained from 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31093317), licensed under a CC-BY license. Not internally tested by Novus Biologicals.