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Biglycan Antibody, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB100-55407

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NB100-55407

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for Biglycan Antibody

Immunogen

Biglycan Antibody is made to a peptide with sequence C-DRLAIQFGNYKK corresponding to C-Terminus according to NP_001702.1.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 22427827).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

41.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Biglycan Antibody

Immunocytochemistry/Immunofluorescence: Biglycan Antibody [NB100-55407] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/ER staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/Immunofluorescence: Biglycan Antibody [NB100-55407] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for Biglycan Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

1:10 - 1:500

Peptide ELISA

Detection limit 1:2000
Application Notes
IHC successful usage on human achilles tendons is reported in scientific literature (PMID: 23001016). Use in ELISA reported in scientific literature (PMID:26694746)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using NB100-55407 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Biglycan

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival. This gene is a candidate gene for the Happle syndrome.

Alternate Names

BGN, DSPG1, PG-S1, SLRR1A

Entrez Gene IDs

633 (Human); 12111 (Mouse); 25181 (Rat)

Gene Symbol

BGN

UniProt

Additional Biglycan Products

Product Documents for Biglycan Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Biglycan Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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