beta-Catenin Antibody (GT2169) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13530
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (99%), Canine (99%), Chicken (97%), Porcine (99%), Rat (100%), Rhesus Macaque (100%), Sheep (99%), Xenopus (97%), Zebrafish (95%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # GT2169
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human beta-Catenin. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
Centrifuge briefly prior to opening.
Scientific Data Images for beta-Catenin Antibody (GT2169) - Azide and BSA Free
![Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530]](https://resources.bio-techne.com/images/products/beta-Catenin-Antibody-GT2169-Western-Blot-NBP3-13530-img0005.jpg "Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530]")
Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530]
Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.![Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530]](https://resources.bio-techne.com/images/products/beta-Catenin-Antibody-GT2169-Western-Blot-NBP3-13530-img0004.jpg "Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530]")
Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530]
Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Wild-type (WT) and beta-Catenin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] -
Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP3-13530) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Applications for beta-Catenin Antibody (GT2169) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: beta-Catenin
Alternate Names
bCatenin, CTNNB1
Gene Symbol
CTNNB1
Additional beta-Catenin Products
Product Documents for beta-Catenin Antibody (GT2169) - Azide and BSA Free
Product Specific Notices for beta-Catenin Antibody (GT2169) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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