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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SAP7F407

Concentration

1.0 mg/ml

Product Summary for Bassoon Antibody (SAP7F407)

Immunogen

Recombinant rat Bassoon.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This affinity purified detects an ~400 kDa protein, corresponding to the apparent molecular mass of Bassoon on SDSPAGE immunoblots, in samples from mouse and rat origins. Additional bands between 97 and 400 kDa may also be detected.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

400 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bassoon Antibody (SAP7F407)

Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.
Immunocytochemistry/Immunofluorescence: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat Hippocampal Neuron, image courtesy of customer.
Immunohistochemistry: Bassoon Antibody (SAP7F407) [NB120-13249] - Bassoon localization in the rat brain cerebellar molecular layer using Bassoon Monoclonal Antibody.

Applications for Bassoon Antibody (SAP7F407)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2.5 ug

Western Blot

1:500
Application Notes
Additional bands between 97 and 400 kDa are also identified and are reported to be proteolytic degradation products of Bassoon. Optimal dilutions/concentrations should be determined by the end user. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 28821669).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB120-13249 in the following applications:

Published Applications

Read 14 publications using NB120-13249 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bassoon

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Alternate Names

bassoon (presynaptic cytomatrix protein)

Entrez Gene IDs

12217 (Mouse); 29138 (Rat)

Gene Symbol

BSN

UniProt

Product Documents for Bassoon Antibody (SAP7F407)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Bassoon Antibody (SAP7F407)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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