BACE-1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77307
![Western Blot: BACE-1 AntibodyBSA Free [NBP1-77307]](https://resources.bio-techne.com/images/products/BACE-1-Antibody-Western-Blot-NBP1-77307-img0007.jpg "Western Blot: BACE-1 AntibodyBSA Free [NBP1-77307]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (94%), Guinea Pig (94%), Rat (94%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BACE. The immunogen is located within the last 50 amino acids of BACE.
Specificity
Human BACE-1 has 6 isoforms, including isoform A (501aa, 55.8kD), isoform B (476aa, 52.9kD), isoform C (457aa, 51.1kD), isoform D (432aa, 48.2kD), isoform 5 (401aa, 45kD) and isoform 6 (376aa, 42.2kD). This antibody detects all human isoforms. Mouse BACE-1 has only 1 isoform (501aa, 55.7kD). Rat BACE-1 has only one isoform identified so far (501aa, 55.8kD).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BACE-1 Antibody - BSA Free
Immunohistochemistry: Rabbit Polyclonal BACE-1 Antibody [NBP1-77307] -
Immunohistochemistry: Rabbit Polyclonal BACE-1 Antibody [NBP1-77307] - Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling BACE with 2253 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Immunohistochemistry: Rabbit Polyclonal BACE-1 Antibody [NBP1-77307] -
Immunohistochemistry Validation of BACE in Human Liver. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-BACE antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western Blot: Rabbit Polyclonal BACE-1 Antibody [NBP1-77307] -
WB Validation in Human Cell Lines. Loading: 10 ug of lysate Antibodies: BACE 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Applications for BACE-1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Western Blot
1 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BACE-1
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 1
Alternate Names
BACE1
Gene Symbol
BACE1
UniProt
Additional BACE-1 Products
Product Documents for BACE-1 Antibody - BSA Free
Product Specific Notices for BACE-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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