B7-H4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06096

Novus Biologicals, part of Bio-Techne
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NBP1-06096
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all B7-H4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SKGKGNANLEYK corresponding to internal region according to NP_078902.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for B7-H4 Antibody

Western Blot: B7-H4 Antibody [NBP1-06096]

Western Blot: B7-H4 Antibody [NBP1-06096]

Western Blot: B7-H4 Antibody [NBP1-06096] - Staining of HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B). Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D) with antibody at 1 ug/mL and Human pancreas iso 1 (E) lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: B7-H4 Antibody [NBP1-06096]

Immunocytochemistry/ Immunofluorescence: B7-H4 Antibody [NBP1-06096]

Immunocytochemistry/Immunofluorescence: B7-H4 Antibody [NBP1-06096] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane/cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: B7-H4 Antibody [NBP1-06096]

Flow Cytometry: B7-H4 Antibody [NBP1-06096]

Flow Cytometry: B7-H4 Antibody [NBP1-06096] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for B7-H4 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 1 ug/mL
Application Notes
WB: Approx. 28 kDa band observed in human pancrease lysates (calculated MW of 30.9 kDa band according to NP_078902.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: B7-H4

B7H4 is a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7H4 deficient mice are only minimally affected, thus suggesting B7H4 is important in the fine tuning of the T-cell mediated immune response. B7H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7H4 could be a useful prognostic marker in Renal Cell Carcinoma (RCC). There are three different isoforms.

Long Name

B7 Homolog 4

Alternate Names

B7H4, B7S1, B7x, Vtcn1

Entrez Gene IDs

79679 (Human); 242122 (Mouse); 295322 (Rat)

Gene Symbol

VTCN1

UniProt

NP_078902.2 (Human)

Additional B7-H4 Products

Product Documents for B7-H4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for B7-H4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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