B7-H3/CD276 Antibody (23GB5035)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51327
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB5035
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human B7-H3/CD276 (Uniprot: Q5ZPR3)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for B7-H3/CD276 Antibody (23GB5035)
Immunocytochemistry/ Immunofluorescence: B7-H3/CD276 Antibody (23GB5035)[NBP3-51327] -
Immunocytochemical staining of Hela cells with B7-H3/CD276 antibody (1:1,000) . Nuclei were stained blue with DAPI; B7-H3/CD276 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
Flow Cytometry: B7-H3/CD276 Antibody (23GB5035)[NBP3-51327] -
Flow cytometric analysis of B7-H3/CD276expression in HeLa cells using B7-H3/CD276 antibody (1:2,000). Green, isotype control; red, CD276.
Western Blot: B7-H3/CD276 Antibody (23GB5035)[NBP3-51327] -
Western blotting analysis using B7-H3/CD276 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for B7-H3/CD276 Antibody (23GB5035)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: B7-H3
Long Name
B7 Homolog 3
Alternate Names
B7H3, CD276
Gene Symbol
CD276
Additional B7-H3 Products
Product Documents for B7-H3/CD276 Antibody (23GB5035)
Product Specific Notices for B7-H3/CD276 Antibody (23GB5035)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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