Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2C5

Concentration

1 mg/ml

View all B7-H2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ICOSLG (NP_056074) produced in HEK293T cell.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34131111). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for B7-H2/ICOSLG Antibody (OTI2C5)

Western Blot: B7-H2/ICOSLG Antibody (OTI2C5) [NBP2-46011]

Western Blot: B7-H2/ICOSLG Antibody (OTI2C5) [NBP2-46011]

Western Blot: B7-H2/ICOSLG Antibody (2C5) [NBP2-46011] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICOSLG.
Immunohistochemistry: B7-H2/ICOSLG Antibody (OTI2C5) [NBP2-46011]

Immunohistochemistry: B7-H2/ICOSLG Antibody (OTI2C5) [NBP2-46011]

Immunohistochemistry: B7-H2/ICOSLG Antibody (2C5) [NBP2-46011] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: B7-H2/ICOSLG Antibody (OTI2C5) [NBP2-46011]

Immunohistochemistry: B7-H2/ICOSLG Antibody (OTI2C5) [NBP2-46011]

Immunohistochemistry: B7-H2/ICOSLG Antibody (2C5) [NBP2-46011] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for B7-H2/ICOSLG Antibody (OTI2C5)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: B7-H2

B7-RP1 is a 40 kD protein, also known as ICOS ligand, B7-H2, and B7h. It has recently been clustered as CD275. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells and macrophages. The expression of this antigen is increased by TNF- alpha stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.

Long Name

B7 Homolog 2

Alternate Names

B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG

Gene Symbol

ICOSLG

Additional B7-H2 Products

Product Documents for B7-H2/ICOSLG Antibody (OTI2C5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for B7-H2/ICOSLG Antibody (OTI2C5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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