Avidin Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB120-7235
Key Product Details
Species Reactivity
Chicken
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Avidin (Hen Egg White)
Reactivity Notes
No cross reactivity occurs against Streptavidin
Specificity
No cross reactivity occurs against Streptavidin.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, as well as purified and partially purified Avidin [Hen Egg White].
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, as well as purified and partially purified Avidin [Hen Egg White].
Scientific Data Images for Avidin Antibody [Biotin]
Western Blot: Avidin Antibody [Biotin] [NB120-7235] - Lane 1: Avidin. Lane 2: None. Load: 100 ng per lane. Used at a dilution of 1:1000 for 1H at RT.
Applications for Avidin Antibody [Biotin]
Application
Recommended Usage
ELISA
1:10000-1:50000
Immunohistochemistry
1:1000-1:5000
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:2000-1:10000
Application Notes
This product has been tested by ELISA and western blot and is suitable for immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Avidin
Alternate Names
AVD, LOC396260
UniProt
Additional Avidin Products
Product Documents for Avidin Antibody [Biotin]
Product Specific Notices for Avidin Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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