Avidin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB120-6675
![Western Blot: Avidin Antibody [NB120-6675]](https://resources.bio-techne.com/images/products/Avidin-Antibody-Western-Blot-NB120-6675-img0001.jpg "Western Blot: Avidin Antibody [NB120-6675]")
Key Product Details
Species Reactivity
Chicken
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Avidin (Hen Egg White)
Reactivity Notes
No cross reactivity occurs against Streptavidin
Specificity
No cross reactivity occurs against Streptavidin.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Avidin Antibody - BSA Free
Western Blot: Avidin Antibody [NB120-6675]
Western Blot: Avidin Antibody [NB120-6675] - Lane 1: Avidin. Lane 2: None. Load: 100 ng per lane. Used at a dilution of 1:1000 for 1H at RT. Analysis using the Biotin conjugated antibody.
Avidin Antibody
Western blot of Avidin Antibody. Lane 1: Molecular Weight Marker. Lane 2: Avidin. Lane 3: Streptavidin. Primary Antibody: Anti-Avidin at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRPApplications for Avidin Antibody - BSA Free
Application
Recommended Usage
ELISA
1:2000-1:20000
Immunohistochemistry
1:1000-1:5000
Immunohistochemistry-Paraffin
1:1000-1:5000
Western Blot
1:1000-1:10000
Application Notes
This product has been tested by western blot and is suitable for ELISA and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 21321260)
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 21321260)
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Avidin
Alternate Names
AVD, LOC396260
UniProt
Additional Avidin Products
Product Documents for Avidin Antibody - BSA Free
Product Specific Notices for Avidin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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