ATF4 Antibody (S360A-24) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42176
![Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176]](https://resources.bio-techne.com/images/products/ATF4-Antibody-S360A-24-Western-Blot-NBP2-42176-img0004.jpg "Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # S360A-24
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5.
Localization
Cytoplasm , Cell Membrane , Nucleus
Specificity
Less then 50% identity with ATF5.Detects approx 60kDa. Does not cross-react with ATF5.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for ATF4 Antibody (S360A-24) - BSA Free
Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176]
Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176] - Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4protein using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).![Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]](https://resources.bio-techne.com/images/products/ATF4-Antibody-S360A-24-Immunocytochemistry-Immunofluorescence-NBP2-42176-img0005.jpg "Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]")
Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]
Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ATF4Antibody (D) Composite.![Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]](https://resources.bio-techne.com/images/products/ATF4-Antibody-S360A-24-Immunocytochemistry-Immunofluorescence-NBP2-42176-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]")
Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]
Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cell Membrane. Magnification: 60X.Applications for ATF4 Antibody (S360A-24) - BSA Free
Application
Recommended Usage
Western Blot
1:1000
Application Notes
1 ug/ml of ATF4 Antibody was sufficient for detection of ATF4 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATF4
Long Name
Activating Transcription Factor 4
Alternate Names
TAXREB67, TXREB
Gene Symbol
ATF4
UniProt
Additional ATF4 Products
Product Documents for ATF4 Antibody (S360A-24) - BSA Free
Product Specific Notices for ATF4 Antibody (S360A-24) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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