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Aspartate Aminotransferase Antibody (23GB5585)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51357

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NBP3-51357-100ul
NBP3-51357-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB5585

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human Aspartate Aminotransferase (Uniprot: P17174)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aspartate Aminotransferase Antibody (23GB5585)

Aspartate Aminotransferase Antibody (23GB5585)

Flow Cytometry: Aspartate Aminotransferase Antibody (23GB5585)[NBP3-51357] -

Validation of Aspartate Aminotransferase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Aspartate Aminotransferase antibody (1:2,000) and analyzed using BD flow cytometer.
Aspartate Aminotransferase Antibody (23GB5585)

Flow Cytometry: Aspartate Aminotransferase Antibody (23GB5585)[NBP3-51357] -

Flow cytometric analysis of Aspartate Aminotransferaseexpression in C2C12 cells using Aspartate Aminotransferase antibody (1:2,000). Green, isotype control; red, Glutamic-oxaloacetic transaminase 1.
Aspartate Aminotransferase Antibody (23GB5585)

Western Blot: Aspartate Aminotransferase Antibody (23GB5585)[NBP3-51357] -

Western blotting analysis using Aspartate Aminotransferase antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Aspartate Aminotransferase Antibody (23GB5585)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aspartate Aminotransferase

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Long Name

Glutamic-oxaloacetic transaminase 1

Alternate Names

aspartate aminotransferase, cytoplasmic, AST1, ASTQTL1, CASPAT, CCAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, EC 2.6.1.1, EC 2.6.1.3, GIG18, Glutamate oxaloacetate transaminase 1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1), GOT1, growth-inhibiting protein 18, SGOT, Transaminase A

Gene Symbol

GOT1

Additional Aspartate Aminotransferase Products

Product Documents for Aspartate Aminotransferase Antibody (23GB5585)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Aspartate Aminotransferase Antibody (23GB5585)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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