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Anti- Adalimumab (Anti-Idiotype) Antibody

Bio-Techne includes R&D Systems | Catalog # MAB9616

Recombinant Monoclonal Antibody.
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MAB9616-100
MAB9616-SP

Key Product Details

Species Reactivity

Multi-Species

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2235F

Product Summary for Anti- Adalimumab (Anti-Idiotype) Antibody

Immunogen

Full length Adalimumab

Specificity

Detects Adalimumab in Western blots and direct ELISAs. In direct ELISAs, no cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Trastuzumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anti- Adalimumab (Anti-Idiotype) Antibody

Detection of Adalimumab antibody by Western Blot.

Detection of Adalimumab by Western Blot.

Western blot shows Cetuximab, Rituximab Trastuzumab, and Adalimumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Adalimumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9616) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Adalimumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in Antibody in ELISA Capture Assay.

Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in ELISA Capture Assay.

Antibody in ELISA Capture Assay. Biotinylated biosimilars were serially diluted and incubated with Anti-Adalimumab (Anti-Idiotype) Antibody (Catalog # MAB9616, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated-IgG control (orange line) was observed.
Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in Direct Antibody in ELISA.

Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in Direct ELISA.

A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Adalimumab (Anti-Idiotype) Antibody (Catalog # MAB9616, 333 ng/mL) detected Adalimumab. No cross-reactivity with Cetuximab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.

Applications for Anti- Adalimumab (Anti-Idiotype) Antibody

Application
Recommended Usage

ELISA

Detects Adalimumab in direct ELISAs.

Western Blot

0.5 µg/mL
Sample: Adalimumab under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adalimumab

The anti-Adalimumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Adalimumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Additional Adalimumab Products

Product Documents for Anti- Adalimumab (Anti-Idiotype) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Anti- Adalimumab (Anti-Idiotype) Antibody

For research use only

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