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Aminopeptidase N/CD13 Antibody (WM15) - BSA Free

Catalog # NB110-58720 | Novus Biologicals a Bio-Techne Brand
Clone WM15 was used by HLDA to establish CD designation.

Key Product Details

Species Reactivity

Human, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # WM15

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for Aminopeptidase N/CD13 Antibody (WM15) - BSA Free

Immunogen

Human AML cells (NM_001150.2).

Specificity

The antibody WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors. HLDA III; WS Code M 213 HLDA IV; WS Code M 44 HLDA IV; WS Code M 209 HLDA V; WS Code M MA191

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aminopeptidase N/CD13 Antibody (WM15) - BSA Free

Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PerCP.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD13 Purified (filled histogram) followed by Anti-Mouse IgG FITC. Cells in the monocyte (left) and granulocyte (right) gates were used for analysis.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [NB110-58720] - Surface staining of human peripheral blood leukocytes with anti-CD13.

Applications for Aminopeptidase N/CD13 Antibody (WM15) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Immunoprecipitation

1:50
Application Notes
Functional: The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates. Purity is > 95% (by SDS-PAGE). This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Aminopeptidase N/CD13

CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

Alternate Names

Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN

Gene Symbol

ANPEP

Additional Aminopeptidase N/CD13 Products

Product Documents for Aminopeptidase N/CD13 Antibody (WM15) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aminopeptidase N/CD13 Antibody (WM15) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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