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AMFR/gp78 Antibody (HL2866)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48741

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-48741

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2866

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragment of human AMFR/gp78.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit AMFR/gp78 Antibody (HL2866) (NBP3-48741) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for AMFR/gp78 Antibody (HL2866)

AMFR/gp78 Antibody (HL2866)

Immunocytochemistry/ Immunofluorescence: AMFR/gp78 Antibody (HL2866) [NBP3-48741] -

AMFR/gp78 antibody [HL2866] detects AMFR/gp78 protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: AMFR/gp78 stained by AMFR/gp78 antibody [HL2866] (NBP3-48741) diluted at 1:500.
Blue: Fluoroshield with DAPI .
AMFR/gp78 Antibody (HL2866)

Western Blot: AMFR/gp78 Antibody (HL2866) [NBP3-48741] -

Boiled and unboiled mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR/gp78 antibody [HL2866] (NBP3-48741) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
AMFR/gp78 Antibody (HL2866)

Western Blot: AMFR/gp78 Antibody (HL2866) [NBP3-48741] -

Unboiled HepG2 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR/gp78 antibody [HL2866] (NBP3-48741) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for AMFR/gp78 Antibody (HL2866)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMFR/gp78

AMFR (Autocrine motility factor receptor, isoform 2) is specific receptor for the autocrine motility factor. AMFR possesses ubiquitin ligase activity and can target both itself and other proteins including CD3D and APOB for proteasomal degradation. AMFR mediates tumor invasion and metastasis. The protein interacts with UBE2G2/UBC7 through a region distinct from the RING finger.

Long Name

Autocrine Motility Factor Receptor

Alternate Names

gp78, RNF45

Gene Symbol

AMFR

Additional AMFR/gp78 Products

Product Documents for AMFR/gp78 Antibody (HL2866)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AMFR/gp78 Antibody (HL2866)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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