Alkaline Phosphatase/ALPP Antibody (8B6) [Allophycocyanin/Cy7]
Novus Biologicals, part of Bio-Techne | Catalog # NB110-3638APCCY7
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Radioimmunodiffusion
Label
Allophycocyanin/Cy7 (Excitation = 650;755 nm, Emission = 767 nm)
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 8B6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28197547). Rat reactivity reported in scientific literature (PMID: 30599898).
Localization
Cell membrane; Lipid-anchor > GPI-anchor
Specificity
Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for Alkaline Phosphatase/ALPP Antibody (8B6) [Allophycocyanin/Cy7]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Radioimmunodiffusion
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Alkaline Phosphatase/ALPP
Long Name
Alkaline Phosphatase, Placental Type
Alternate Names
Alkaline Phosphatase, PLAP, SEAP
Gene Symbol
ALPP
Additional Alkaline Phosphatase/ALPP Products
Product Documents for Alkaline Phosphatase/ALPP Antibody (8B6) [Allophycocyanin/Cy7]
Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (8B6) [Allophycocyanin/Cy7]
Cy is a registered trademark of Global Life Sciences Solutions Germany GmbH.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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