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Ago2/eIF2C2 Antibody (23GB1790)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51777

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NBP3-51777-100ul
NBP3-51777-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1790

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human Ago2/eIF2C2 (Uniprot: Q9UKV8)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ago2/eIF2C2 Antibody (23GB1790)

Ago2/eIF2C2 Antibody (23GB1790)

Immunocytochemistry/ Immunofluorescence: Ago2/eIF2C2 Antibody (23GB1790)[NBP3-51777] -

Ago2/eIF2C2 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: Argonaute RISC catalytic component 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Argonaute RISC catalytic component 2 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
Ago2/eIF2C2 Antibody (23GB1790)

Immunocytochemistry/ Immunofluorescence: Ago2/eIF2C2 Antibody (23GB1790)[NBP3-51777] -

Immunocytochemical staining of C2C12 cells with Ago2/eIF2C2 antibody (1:1,000). Nuclei were stained blue with DAPI; Ago2/eIF2C2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Ago2/eIF2C2 Antibody (23GB1790)

Flow Cytometry: Ago2/eIF2C2 Antibody (23GB1790)[NBP3-51777] -

Flow cytometric analysis of Ago2/eIF2C2expression in C2C12 cells using Ago2/eIF2C2 antibody (1:2,000). Green, isotype control; red, Argonaute RISC catalytic component 2.

Applications for Ago2/eIF2C2 Antibody (23GB1790)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ago2/eIF2C2

Ago2 / eIF2C2 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Alternate Names

AGO2argonaute 2, Argonaute2, EC 3.1.26.n1, EC 3.1.26.n2, eIF2C 2, eIF-2C 2, Eukaryotic translation initiation factor 2C 2, eukaryotic translation initiation factor 2C, 2, hAgo2, MGC3183, PAZ Piwi domain protein, PPD, protein argonaute-2, Protein slicer, Q10

Gene Symbol

AGO2

Additional Ago2/eIF2C2 Products

Product Documents for Ago2/eIF2C2 Antibody (23GB1790)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ago2/eIF2C2 Antibody (23GB1790)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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