Actin (Muscle Specific) Antibody (3G8C7A6) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43563
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3G8C7A6
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by immunizing BALB/c mice with a recombinant human actin protein. (Uniprot# P68133)
Specificity
This antibody binds actin. Actin is a type of spherical multi-functional protein that forms a microfilament. Actin is widely existent in eukaryotic organisms, and there is a major component constituting the muscle segment and the cell skeleton in the cytoplasm and cellular nucleus of the intramuscular cell. Muscle specific actin (MSA) is a highly conserved, ubiquitous protein found in muscle and some non-muscle cells. In addition to participating in the normal morphology of maintaining cells, actin also participates in the formation, adhesion, migration, apoptosis, material transport, transmembrane information transfer, and receptor aggregation.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Scientific Data Images for Actin (Muscle Specific) Antibody (3G8C7A6) - Chimeric - BSA Free
Western Blot: Actin (Muscle Specific) Antibody (3G8C7A6) - Chimeric - BSA Free [NBP3-43563] -
Western Blot: Actin (Muscle Specific) Antibody (3G8C7A6) - Chimeric - BSA Free [NBP3-43563] - HEK293 (A) (1µg/ml), HeLa (B) (0.1µg/ml) cells lysate, and human skeletal muscle (C) (0.01µg/ml) tissue lysate (35µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with NBP3-43563 at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.
Flow Cytometry: Actin (Muscle Specific) Antibody (3G8C7A6) - Chimeric - BSA Free [NBP3-43563] -
Flow Cytometry: Actin (Muscle Specific) Antibody (3G8C7A6) - Chimeric - BSA Free [NBP3-43563] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or NBP3-43563 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.
Immunocytochemistry/ Immunofluorescence: Actin (Muscle Specific) Antibody (3G8C7A6) - Chimeric - BSA Free [NBP3-43563] -
Immunocytochemistry/ Immunofluorescence: Actin (Muscle Specific) Antibody (3G8C7A6) - Chimeric - BSA Free [NBP3-43563] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on coverslips stained with NBP3-43563 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43563, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.Applications for Actin (Muscle Specific) Antibody (3G8C7A6) - Chimeric - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Actin (Muscle Specific)
Alternate Names
ACTA, ACTC, actin, alpha 2, smooth muscle, aorta, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, aortic smooth muscle, ACTSA, alpha-cardiac actin, ASD5, ASMA, Cell growth-inhibiting gene 46 protein, CFTD, CFTD1, CFTDM, CMD1R, CMH11, MPFD, NEM1, NEM2, NEM3, nemaline myopathy type 3
Gene Symbol
ACTA1
Additional Actin (Muscle Specific) Products
Product Documents for Actin (Muscle Specific) Antibody (3G8C7A6) - Chimeric - BSA Free
Product Specific Notices for Actin (Muscle Specific) Antibody (3G8C7A6) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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